Kit's description
SDS-PAGE is one of the most powerful and commonly used techniques in molecular biology. However, traditional protein gel staining and destaining methods take several hours to finish and leaves a high blue background on the gel reducing the detection sensitivity. BioVision’s Gel-FASTTM 20 Minutes Gel Staining/Destaining Kit provides a fast and sensitive method for SDS-PAGE gel staining and destaining. The procedure requires only 5 minutes for gel staining and 15 minutes for destaining. A total of 20 minutes allow visualization of as little as 5 ng of protein on a water-clear background. The kit contains sufficient solutions to stain and destain up to 40 pieces of protein mini gels.
Highlights
• Detection method: Gel Documentation machine, Naked eye • Species reactivity: N/A • Application: This kit provides a fast and sensitive method for SDS:PAGE gel staining and destaining
Kit's benefits
• The kit contains unique formulations of reagents for fast and convenient staining/destaining of SDS-PAGE gel. A total of 20 minutes (5 min staining and 15 min destaining) allows visualization of as little as 5 ng of protein on a water-clear background.
Category
Gel Staining/Destaining
Contents
• 2X Solution A • 6X Solution B
Shipping condition
gel pack
Maximum time can be stored
12 months
Species reactivity
see datasheet
Samples tested
Crude and purified proteins from various sources
Test
A gel is a solid jelly-like material that can have properties ranging from soft and weak to hard and tough. Gels are defined as a substantially dilute cross-linked system, which exhibits no flow when in the steady-state. By weight, gels are mostly liquid, yet they behave like solids due to a three-dimensional cross-linked network within the liquid. It is the crosslinking within the fluid that gives a gel its structure (hardness) and contributes to the adhesive stick (tack). In this way gels are a dispersion of molecules of a liquid within a solid in which the solid is the continuous phase and the liquid is the discontinuous phase. The word gel was coined by 19th-century Scottish chemist Thomas Graham by clipping from gelatin.