Area of research
Differentiation & Development
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The antispermatozoa antibody ELISA (Enzyme Linked ImmunoSorbent Assay) from IBLAmerica is a solidphase sandwich enzymeimmunoassay for the quantitative determination of antispermatozoa antibodies in human seminal plasma. The ELISAplate is coated with a mix of spermatozoa proteins which are recognized by antispermatozoa antibodies. The samples and standards are pipetted into the wells and then incubated. During this incubation antispermatozoa antibodies bind to the spermatozoa proteins and are thus immobilised on the plate. After washing the enzyme conjugate, consisting of antihuman globulin antibodies covalently coupled to horseradish peroxidase, is added. After removal of the unbound conjugate by washing the horseradish peroxidase oxidizes the then added substrate TMB (3,3’,5,5’tetramethylbenzidine) yielding a colour reaction which is stopped with 0.25 M sulphuric acid (H2SO4). The extinction is measured at a wavelength of 450 nm with a microplate reader. The use of a reference measurement with a wavelength of 550 nm or higher is recommended.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED