Area of research
Cytokines & Growth Factors
Store at 2-8 deg C
Optimal conditions to be determined by end user
An antirat TGFbeta1 coating antibody is adsorbed onto microwells. Rat TGFbeta1 present in the sample or standard binds to antibodies adsorbed to the microwells. A biotinconjugated antirat TGFbeta1 antibody is added and binds to rat TGFbeta1 captured by the first antibody. Following incubation unbound biotin conjugated antirat TGFbeta1 antibody is removed during a wash step. StreptavidinHRP is added and binds to the biotin conjugated antirat TGFbeta1 antibody. Following incubation unbound StreptavidinHRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of rat TGFbeta1 present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 rat TGFbeta1 standard dilutions and rat TGFbeta1 sample concentration determined.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.