Area of research
Cytokines & Growth Factors
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An antirat IL1alpha coating antibody is adsorbed onto microwells. Rat IL1alpha present in the sample or standard binds to antibodies adsorbed to the microwells. A biotinconjugated antirat IL1alpha antibody is added and binds to rat IL1alpha captured by the first antibody. Following incubation unbound biotin conjugated antirat IL1alpha antibody is removed during a wash step. StreptavidinHRP is added and binds to the biotinconjugated anti rat IL1alpha antibody. Following incubation unbound StreptavidinHRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of rat IL1alpha present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 rat IL1alpha standard dilutions and rat IL1alpha sample concentration determined.
The IL1 alpha ELISA kit is a α- or alpha protein sometimes glycoprotein present in blood.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.