Area of research
Hormones & Steroids
Store at 2-8 deg C
Optimal conditions to be determined by end user
This rat Gastrin I kit is a competitive immunoassay for the determination of Gastrin in samples. The kit uses a polyclonal antibody to Gastrin I to bind, in a competitive manner, the Gastrin I in the sample or an alkaline phosphatase molecule which has Gastrin I covalently attached to it. After a simultaneous incubation at room temperature the excess reagents are washed away and substrate is added. After a short incubation time the enzyme reaction is stopped and the yellowcolor generated read on amicroplate reader at 405nm. The intensity of the bound yellow color is inversely proportional to the concentration of Gastrin I in either standards or samples. The measured optical density is used to calculate the concentration of Gastrin I.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.