Research kit

Cardiac Markers

Store at 2-8 deg C

Blue Ice

ELISA

Optimal conditions to be determined by end user

Rat sera for testing are diluted to 1:4,000 and allowed to react with antibodies coated on specially treated microwells. After appropriate incubation, the wells are washed to remove unreacted serum proteins, and an enzymelabeled rabbit antirat CRP (conjugate) is then added to react with and tag the antigenantibody complexes. Following another incubation period, the wells are again washed to remove unreacted conjugate. A urea peroxide substrate with TMB as chromogen is added to start color development. Development of a blue color indicates a positive reaction while negative reactions appear colorless or with a trace of blue. The reaction is interrupted with a stop solution that turns the blue positive reactions to yellow. Negative reactions remain colorless or with a hint of yellow. Color intensity (absorbance) is read at a wavelength of 450nm on a spectrophotometer or ELISA reader. Assigning a value for the absorbance can be accomplished by the use of a standard curve generated by measuring twofold dilutions of the standard provided.

C reactive proteins are detected by anti-CRP antibodies supplied by GENTAUR.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Rattus norvegicus