Form & Buffer
This Kit contains: Microplate reader in standard size.2. Automated plate washer, Adjustable pipettes and pipette tips. Multichannel pipettes are reco mMended in the condition of large amount of samples in the detection, Clean tubes and Eppendorf tubes, Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, ,5g Nacl; 450 ul of purified acetic acid or 700 ul of concentrated hydrochloric acid to 1000ml H2O and adjust pH to ,2-,, Finally, adjust the total volume to 1L.Preparation of 0.01 M PBS: Add ,5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to ,2-,, Finally, adjust the total volume to 1L. Notice for Application of Kit1． Before using Kit, spin tubes and bring down all components to bottom of tube.2． Duplicate well assay was reco mMended for both standard and sample testing.3． Don’t let 96-well plate dry, dry plate will inactivate active components on plate.4． In order to avoid marginal effect of plate incubation due to temperature difference (reaction may be stronger in the marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37℃ for 30 min before using.