All suppliers fitzgerald Pregnenolone ELISA kit

Information

Catalog number
55R-IB59107
Name
Pregnenolone ELISA kit
Supplier
Size
96 wells
Price
376.00
Buy

Details

Category
Research kit
Area of research
Hormones & Steroids
Storage
Store at 2-8 deg C
Shipping conditions
Blue Ice
Tested for
ELISA
Usage Recommendations
Optimal conditions to be determined by end user
Assay Information
The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition  occurs between an unlabeled antigen (present in standards, control and patient samples) and an enzymelabelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The  intensity of the colour formed is inversely proportional to the concentration of pregnenolone in the sample. A set of  standards is used to plot a standard curve from which the amount of pregnenolone in patient samples and controls can  be directly read.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED