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Store at 2-8 deg C
Optimal conditions to be determined by end user
This Prostaglandin E2 (PGE2) kit is a competitive immunoassay for the quantitative determination of Prostaglandin E2 in biological fluids. Please read the complete kit insert before performing this assay. The kit uses a monoclonal antibody to PGE2 to bind, in a competitive manner, the PGE2 in the sample, standard, or an alkaline phosphatase molecule which has PGE2 covalently attached to it. After a simultaneous incubation at room temperature the excess reagents are washed away and substrate is added. After a short incubation time the enzyme reaction is stopped and the yellow color generated read on a microplate reader at 405 nm. The intensity of the bound yellow color is inversely proportional to the concentration of PGE2 in either standards or samples. The measured optical density is used to calculate the concentration of PGE2.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED