Store at 2-8 deg C
Optimal conditions to be determined by end user
This mouse TNFalpha Enzyme Immunometric Assay (EIA) kit is a complete kit for the determintation of mouse TNFalpha in biological fluids. Please read the complete kit insert before performing this assay. The kit uses a monoclonal antibody to mouse TNFalpha immobilized on a microtiter plate to bind the mouse TNFalpha in the standards or sample. A recombinant mouse TNFalpha Standard is provided in the kit. After a short incubation the excess sample or standard is washed out and a polyclonal antibody to mouse TNFalpha is added. This antibody binds to the mouse TNFalpha captured on the plate. After a short incubation the excess antibody is washed out and donkey antirabbit IgG conjugated to Horseradish peroxidase is added, which binds to the polyclonal mouse TNFalpha antibody. Excess conjugate iswashed out and substrate is added.After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of mouse TNFalpha in either standards or samples.
The Mouse TNF alpha ELISA kit is a α- or alpha protein sometimes glycoprotein present in blood.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.