Area of research
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An antimonkey sPselectin coating antibody is adsorbed onto microwells. Monkey sPselectin present in the sample or standard binds to antibodies adsorbed to the microwells. A HRPconjugated antimonkey sPselectin antibody is added and binds to monkey sPselectin captured by the first antibody. Following incubation unbound HRP conjugated antimonkey sPselectin is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of monkey sPselectin present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 monkey sPselectin standard dilutions and monkey sPselectin concentration determined.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Rhesus Monkeys proteins are often measured by ELISA on serum or plasma since consensus epitopes with the human ELISA are used for producing the antibodies of these ELISA test kits. Often cDNA of monkeys is used as alternative to human cDNA as a model for drug development. Monkeys are generally considered to be intelligent, particularly Old World monkeys.