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The Influenza A IgM antibody test kit is based on the principle of the enzyme immunoassay (EIA). Influenza A antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-touse standards are pipetted into the wells of the microtiter plate. A binding between the IgM antibodies of the serum and the immobilized Influenza A antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use antihuman-IgM peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgM antibodies is directly proportional to the intensity of the color. NOTE that samples containing hyper-immune levels of anti-human IgG antibodies may cause false positive results in this assay. Any patient samples testing positive should be pre-diluted with IgG adsorbent and re-tested to confirm elevated levels of IgM.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Influenza A and B H1N1 H3N2 Hemagglutinin-nucleoprotein recombinant proteins, peptides and antibodies detect a virus commonly known as "the flu". Influenza is an infectious disease caused by an influenza virus. Symptoms can be mild to severe. The most common symptoms include a high fever, runny nose, sore throat, muscle pains, headache, coughing, and feeling tired. These symptoms typically begin two days after exposure to the virus and most last less than a week. The cough, however, may last for more than two weeks. In children, there may be nausea and vomiting, but these are not common in adults.