Area of research
Cytokines & Growth Factors
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The ELISA for IGFBP2 utilizes a specific high affinity polyclonal antibody and a specific monoclonal antibody for this protein. It recognizes quantitatively IGFBP2 and is unaffected by excess of IGFl or IGFII levels. Related molecules such as lGFBP3 show no crossreaction in the assay. The standards are also prepared of recombinant IGFBP2 in the range of 1 to 80 ng/ml. Detailed specification of antibody and pure IGFBP2 (data sheets) can be ordered from Mediagnost GmbH. Chromatographically purified IGFBP2, as well as IGFBP2antiserum are available at Mediagnost GmbH also as research reagents. Different human sera were spiked with recombinant human IGFBP2:recovery was found between 97% and 115%. The theoretical sensitivity of the assay is 0.2 ng/ml (2 x SD of zero standard). Intraassay and interassay variation coefficients were found both <10%.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED