Area of research
Cytokines & Growth Factors
Store at 2-8 deg C
Optimal conditions to be determined by end user
The principle of the following enzyme immunoassay test follows a typical twostep capture or ‘sandwich’ type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for IGFBP1 is immobilized onto the microwell plate and another monoclonal antibody specific for a different region of IGFBP1 is conjugated to horse radish peroxidase (HRP). IGFBP1 from the sample and standards are allowed to bind to the plate, washed, and subsequently incubated with the HRP conjugate. After a second washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed by the enzymatic reaction is directly proportional to the concentration of IGFBP1 in the sample. A set of standards is used to plot a standard curve from which the amount of IGFBP1 in patient samples and controls can be directly read.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED