Assay Information
This human XIAP Enzyme Immunometric Assay (EIA) kit is a complete kit for the determination of XIAP in human cell lysates. Please read the complete kit insert before performing this assay. The kit uses a monoclonal antibody to human XIAP immobilized on a microtiter plate to bind the XIAP in the standards or sample. Arecombinant human XIAP Standard is provided in the kit. After a short incubation the excess sample or standard is washed out and a biotinylated monoclonal antibody to human XIAP is added. This antibody binds to the XIAP captured on the plate. After a short incubation the excess antibody is washed out and streptavidin conjugated to Horseradish peroxidase is added, which binds to the biotinylated monoclonal XIAP antibody. Excess conjugate is washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of human XIAP in either standards or samples.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.