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Optimal conditions to be determined by end user
An antihuman TNF alpha coating antibody is adsorbed onto microwells. Human TNF alpha present in the sample or standard binds to antibodies adsorbed to the microwells. A biotinconjugated antihuman TNF alpha antibody is added and binds to human TNF alpha captured by the first antibody. Following incubation unbound biotinconjugated antihuman TNF alpha antibody is removed during a wash step. StreptavidinHRP is added and binds to the biotinconjugated antihuman TNF alpha antibody. Following incubation unbound StreptavidinHRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of human TNF alpha present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 human TNF alpha standard dilutions and human TNF alpha sample concentration determined.
The TNF alpha ELISA kit is a α- or alpha protein sometimes glycoprotein present in blood.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.