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The IBLAmerica human Myeloperoxidase (MPO) Enzyme Immunometric Assay (EIA) kit is complete kit for the determination ofMPO in biological fluids. Please read the complete kit insert before performing this assay. The kit uses a monoclonal antibody to MPO immobilized on amicrotiter plate to bind theMPO in the standards or sample. A native MPO Standard is provided in the kit. After a short incubation, the excess sample or standard is washed out and a rabbit polyclonal antibody to MPO is added. This antibody binds to the MPO captured on the plate. After a short incubation, the excess antibody is washed out and goat antirabbit IgG conjugated to horseradish peroxidase is added, which binds to the polyclonal MPO antibody. Excess conjugate is washed out and substrate is added.After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of MPO in either standards or samples.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED