Area of research
Hormones & Steroids
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Optimal conditions to be determined by end user
This ELISA for IGFI E20 is a socalled SandwichAssay using two specific and high affinity antibodies. The IGFI in the samples binds to the first antibody coated on the microtiterplate, the second specific antiIGFIantibody binds in turn to the immobilised IGFI. The second antibody is biotinylated, the subsequently incubated StreptavidinperoxidaseEnzyme Conjugate will bind to it, and thus in the final substrate incubation step colour development will be catalysed quantitatively depending on the IGFIlevel of the samples. The Standards of the ELISA E20 are prepared from recombinant IGFI in concentrations of 1, 5, 15, 30 and 50 ng/ml.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED