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The IBLAmerica human Complement C4a des Arg kit is a competitive immunoassay for the determination of human C4a desArg in plasma. Please read the complete kit insert before performing this assay. The kit uses a polyclonal antibody to human C4a des Arg to bind, in a competitive manner, the human C4a des Arg in the standard or sample or an alkaline phosphatase molecule which has human C4a des Arg attached to it. After incubation at room temperature the excess reagents are washed away and substrate is added. After a short incubation, the enzyme reaction is stopped and the yellow color generated is read on a microplate reader at 405nm. The intensity of the bound yellow color is inversely proportional to the concentration of human C4a des Arg in either standards or samples. The measured optical density is used to calculate the concentration of human C4a des Arg.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED