All suppliers fitzgerald Human Bax ELISA kit


Catalog number
Human Bax ELISA kit
96 wells


Research kit
Area of research
Cell Cycle & Cell Death
Store at 2-8 deg C
Shipping conditions
Blue Ice
Tested for
Usage Recommendations
Optimal conditions to be determined by end user
Assay Information
This human Bax Enzyme Immunometric Assay (EIA) kit is a complete kit for the determination of Bax in human cell lysates. Please read the complete kit insert before performing this assay. The kit uses a monoclonal antibody to Bax immobilized on a microtiter plate to bind the Bax in the standards or sample. A recombinant human Baxalpha Standard is provided in the kit. After a short incubation, the excess sample or standard iswashed out and a biotinylated monoclonal antibody to Bax is added. This antibody binds to the Bax captured on the plate. After a short incubation, the excess antibody is washed out and Streptavidin conjugated to Horseradish peroxidase is added, which binds to the biotinylated monoclonal Bax antibody. Excess conjugate is washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of Bax in either standards or samples.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED