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This assay is a solid phase enzymelinked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with an antibody, directed towards an epitope of an antigen molecule. An aliquot of patient serum is incubated in the coated well with enzyme conjugated second antibody (EAb), directed towards a different region of the antigen molecule. After incubation the unbound EAb is washed off. The amount of bound EAb is proportional to the concentration of antigen in the sample. After adding the substrate solution, the intensity of colour developed is proportional to the antigen concentration in the sample. The measured ODs of the standards are used to construct a calibration curve against which the unknown samples are calculated.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED