Area of research
Nutrition & Metabolism
Store at 2-8 deg C
Optimal conditions to be determined by end-user
AntiGliadin IgG is an indirect solid phase enzyme immunometric assay (ELISA). It is designed for the quantitative measurement of IgG class antibodies directed against Gliadin. Preparations of purified Gliadin from wheat are coated on the microplates. The microplates can be divided into 12 modules of 8 wells each or can be used completely for 96 determinations. Each well can be separated from the module ("breakaway"). The binding of present antibodies, formation of the sandwich complexes and enzymatic color reaction take place during three different reaction phases. To read the optical density a microplate reader with a 450 nm filter is required. Bichromatic measurement with a 600690 nm reference is recommended.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.