All suppliers fitzgerald Calcitonin ELISA kit


Catalog number
Calcitonin ELISA kit
96 wells


Research kit
Area of research
Hormones & Steroids
Store at 2-8 deg C
Shipping conditions
Blue Ice
Tested for
Usage Recommendations
Optimal conditions to be determined by end user
Assay Information
The Calcitonin Immunoassay is a twosite ELISA [EnzymeLinked ImmunoSorbent Assay] for  the measurement of the biologically intact 32 amino acid chain of Calcitonin. It utilizes two different mouse monoclonal antibodies to human calcitonin specific for welldefined regions on the calcitonin molecule. One antibody binds only to Calcitonin 1123 and this antibody is biotinylated. The other antibody binds only to Calcitonin 2132 and this antibody is labeled with  horseradish peroxidase [HRP] for detection. In this assay, calibrators, controls, or patient samples are simultaneously incubated with the enzyme labeled antibody and a biotin coupled antibody in a streptavidincoated microplate well.  Thus the calcitonin in the sample is “sandwiched” between these two antibodies. At the end of the assay incubation, the microwell is washed to remove unbound components and the enzyme bound to the solid phase is incubated with the substrate, tetramethylbenzidine (TMB). An acidic stopping solution is then added to stop the reaction and converts the color to yellow. The intensity of the yellow color is directly proportional to the concentration of calcitonin in the  sample. A dose response curve of absorbance unit vs. concentration is generated using results obtained from the calibrators. Concentrations of calcitonin present in the controls and patient samples are determined directly from this curve.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED