Area of research
Differentiation & Development
Store at 2-8 deg C
Optimal conditions to be determined by end user
This betacatenin ELISA kit is a complete kit for the determination of betacatenin in cell lysates. Please read the complete kit insert before performing this assay. The kit uses a monoclonal antibody to betacatenin immobilized on a microtiter plate to bind the betacatenin in the standards or sample. A recombinant human betacatenin Standard is provided in the kit. After a short incubation the excess sample or standard is washed out and a polyclonal antibody to betacatenin is added. This antibody binds to the betacatenin captured on the plate. After a short incubation the excess antibody is washed out and Goat antiRabbit IgG conjugated to Horseradish peroxidase is added, which binds to the polyclonal betacatenin antibody. Excess conjugate is washed out and substrate is added. After a short incubation, the enzyme reaction is stopped and the color generated is read at 450 nm. The measured optical density is directly proportional to the concentration of betacatenin in either standards or samples.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED