Store at 2-8 deg C
Optimal conditions to be determined by end-user
An antihuman Bcl2 coating antibody is adsorbed onto microwells. Human Bcl2 present in the sample or standard binds to antibodies adsorbed to the microwells. A biotinconjugated antihuman Bcl2 antibody is added and binds to human Bcl2 captured by the first antibody. Following incubation unbound biotinconjugated antihuman Bcl2 antibody is removed during a wash step. StreptavidinHRP is added and binds to the biotinconjugated antihuman Bcl2 antibody. Following incubation unbound StreptavidinHRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of human Bcl2 present in the sample or standard. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A standard curve is prepared from 7 human Bcl2 standard dilutions and human Bcl2 sample concentration determined.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED