Area of research
Nutrition & Metabolism
Store at 2-8 deg C
Optimal conditions to be determined by end user
This 13hydroxyoctadecadienoic acid [13(S)HODE] Enzyme Immunoassay (EIA) kit is a competitive immunoassay for the quantitative determination of free 13(S)HODE in biological fluids. Please read the complete kit insert before performing this assay. The kit uses a polyclonal antibody to 13(S)HODE to bind, in a competitive manner, the 13(S)HODE in the sample or an alkaline phosphatasemoleculewhich has 13(S)HODEcovalentlyattached to it. After a simultaneous incubation at room temperature the excess reagents are washed away and substrate is added. After another incubation, the enzyme reaction is stopped and the yellowcolor generated read on amicroplate reader at 405 nm. The intensity of the bound yellowcolor is inversely proportional to the concentration of 13(S)HODE in either standards or samples. The measured optical density is used to calculate the concentrationof13(S)HODE.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED